Descripción del título
The 3D Culturing of Organoi...
We describe a protocol to isolate murine small intestinal crypts and culture intestinal 3D organoids from the crypts. Additionally, we describe a method to generate organoids from a single intestinal stem cell in the absence of a sub-epithelial cellular niche.\r\n
Material Proyectable
material_proyectable Rebiun35473700 https://catalogo.rebiun.org/rebiun/record/Rebiun35473700 m o c vz czazuu cr cnannnuuuuu 230725s2023 mau003 o vleng d 65219 JoVE UCEU on1391118668 65219 MyJoVE Corporation MAJOV eng rda pn MAJOV eng eng The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research Cambridge JoVE 2023 Cambridge Cambridge JoVE 1 online resource (1 video file (2 min., 35 sec.)) sound, color 1 online resource (1 video file (2 min., 35 sec.)) 000235 Two-dimensional Moving Image tdi rdacontent computer c rdamedia online resource cr rdacarrier digital rdatr video file rdaft Biology 1940-087X Presented by MyJoVE Corporation We describe a protocol to isolate murine small intestinal crypts and culture intestinal 3D organoids from the crypts. Additionally, we describe a method to generate organoids from a single intestinal stem cell in the absence of a sub-epithelial cellular niche.\r\n Presented in English; subtitles in English Instructional and Educational Work MyJoVE Corporation production company publisher Biology. 1940-087X