Descripción del título
Apoptosis techniques and pr...
The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death
Monografía
monografia Rebiun36935845 https://catalogo.rebiun.org/rebiun/record/Rebiun36935845 m o d cr |n||||||||| 021031s2002 njua ob 001 0 eng c 2002513309 GBA2Z2923 bnb 170925487 227335207 228372303 320960287 648273938 771203470 964932385 967615271 994716135 1005787115 1035705683 1043050975 1078864315 1078867034 1081190483 1087477022 1097299656 1164871564 1166215594 1229792180 1245068537 1264728863 1295495922 1588290123 alk. paper) 9781588290120 alk. paper) 9781592591886 electronic bk.) 1592591884 electronic bk.) 1280821507 9781280821509 9786610821501 661082150X 9781617373060 1617373060 9781489938855 1489938850 10.1385/1592591884. doi NZ1 15212088 COO eng pn COO YUM N$T YDXCP UAB CUV CNTRU GW5XE PUL IDEBK YNG CSU E7B OCLCQ OCLCO OCLCQ OCLCF OCLCQ OCL OCLCQ VT2 OTZ OCLCQ TFH OCLCO BUF STF OCLCQ CASUM MERER OCLCQ U3W OCLCO OCLCQ OCLCO OCLCQ OCLCO OCLCQ YOU LND CANPU OCLCQ OCLCO OCLCA OCLCQ AU@ OCLCQ OCLCO OCLCQ OCLCO OCL GW5XE OCLCA HS0 UWK LVT OCLCQ LUN CN4UL INARC OCLCO OCLCQ OCLCO UKAHL OCLCL OCLCQ pcc SCI 056000 bisacsh PSAN. bicssc PSAN. thema 571.9/36 22 Apoptosis techniques and protocols 2nd ed. edited by Andréa C. Leblanc Totowa, N.J. Humana 2002 Totowa, N.J. Totowa, N.J. Humana 1 online resource (xv, 250 pages) illustrations 1 online resource (xv, 250 pages) Text txt rdacontent computer c rdamedia online resource cr rdacarrier Neuromethods 0893-2336 37 Includes bibliographical references and index Molecular characterization of the proapoptotic protein bax / Yi-Te Hsu and Soraya Smaili -- Assessing cytochrome-c release from mitochondria / Douglas W. Ethell and Douglas R. Green -- Monitoring caspases in neuronal cell death / Younes Bounhar, Omar Tounekti, and Andréa C. LeBlanc -- HSV amplicon vectors in neuronal apoptosis studies / Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff -- Microinjections to study the specific role of proapoptotic proteins in neurons / Yan Zhang and Andréa C. LeBlanc -- Designing microarray experiments for neurobiology / P. Scott Eastman and Jeanne F. Loring -- Analysis of gene expression by nylon membrane cDNA arrays / H. Michael Tucker and Steven Estus -- Glyceraldehyde-3-phosphate dehydrogenase as a target for antiapoptotic drugs / Mark D. Berry and Paula C. Ashe -- Analytical approaches for investigating apoptosis and other biochemical events in compartmented cultures of sympathetic neurons / Barbara Karten [and others] -- Detection and analysis of synaptosis / Greg M. Cole and Karen Gylys -- In situ detection of apoptotic neurons / Kevin A. Roth -- Apoptotic and oxidative indicators in Alzheimer's Disease / Arun K. Raina [and others] The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death UofL: Multi-user access (3 concurrent users) English Apoptosis- Laboratory manuals Apoptosis- physiology Brain Diseases- physiopathology Histocytological Preparation Techniques Neurons- physiology Apoptose- Manuels de laboratoire SCIENCE- Life Sciences- Anatomy & Physiology. Apoptosis. Laboratory Manual Laboratory manuals. Laboratory manuals. Manuels de laboratoire. LeBlanc, Andréa C. Print version Apoptosis techniques and protocols. 2nd ed. Totowa, N.J. : Humana, 2002 1588290123 9781588290120 (DLC) 2002513309 (OCoLC)48836858 Neuromethods 37. 0893-2336