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Antibody engineering / Rola...
Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided: Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radiolabelled recombinant antibodies Bispecific antibodies Antibody - enzyme fusions Intracellular antibodies Determination of affinity and specificity Computer analysis of antibody sequence and structure Epitope analysis by various phage display systems and peptide spot membranes Eukaryotic (plant, baculovirus, yeast, mammalian cells) and prokaryotic production systems for recombinant antibodies Purification systems Xenograft mice Emerging technologies
Monografía
monografia Rebiun38209750 https://catalogo.rebiun.org/rebiun/record/Rebiun38209750 m o d cr cnu---unuuu 131029s2001 gw a obf 001 0 eng d 859587937 1086475295 9783662046050 electronic bk.) 3662046059 electronic bk.) 3540413545 9783540413547 10.1007/978-3-662-04605-0 doi AU@ 000057648915 NZ1 15185007 GW5XE eng rda pn GW5XE NUI OCLCQ OCLCF OCL EBLCP OCLCQ YDX UAB OCLCQ OCLCO OCLCA AU@ OCLCO OCLCA OCLCQ OCLCO LEAUB OCLCQ OCLCA OCLCQ OCLCA UKAHL OCLCO OCLCQ OCLCO OCLCQ OCLCO OCLCL OCLCQ OCLCA QR lcco PSF bicssc SCI049000 bisacsh PSF thema 616.07/98 22 42.03 bcl XD 3111 rvk CIT 960f stub MED 553f stub Antibody engineering Roland Kontermann, Stefan Dübel (eds.). Berlin New York Springer [2001] Berlin New York Berlin New York Springer 2001 1 online resource (xii, 790 pages) illustrations 1 online resource (xii, 790 pages) Text txt rdacontent computer c rdamedia online resource cr rdacarrier text file PDF Springer lab manuals Includes bibliographical references and index 50 chapters describing protocols in the following areas (Part titles): Introduction -- Generation of recombinant antibodies from hybridoma -- Generation of antibody libraries -- Isolation of antibody fragments from combinatorial libraries -- Expression and purification of antibody fragments in bacteria -- Expression and purification of antibody fragments in eukaryotic cells -- Determination of affinities -- Sequence and structure analysis and modelling epitope mapping -- In vivo experiments -- Affinity maturation of antibody fragments -- Humanisation of antibody fragments -- Antibody engineering to improve stability -- Bivalent and bispecific antibody fragments -- Recombinant antibody-fusion proteins -- Intracellular targeting of antibody fragments. The complete table of contents can be found on the Internet: http://www.springer.de Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided: Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radiolabelled recombinant antibodies Bispecific antibodies Antibody - enzyme fusions Intracellular antibodies Determination of affinity and specificity Computer analysis of antibody sequence and structure Epitope analysis by various phage display systems and peptide spot membranes Eukaryotic (plant, baculovirus, yeast, mammalian cells) and prokaryotic production systems for recombinant antibodies Purification systems Xenograft mice Emerging technologies Recombinant antibodies- Laboratory manuals Antibodies- genetics DNA, Recombinant- immunology Antibody Formation- genetics Genetic Engineering- methods Anticorps recombinants- Manuels de laboratoire Recombinant antibodies. Aufsatzsammlung. Herstellung. Labormedizin. Labortechnik. Rekombinantes Protein. Antikörper. Genetische manipulatie. Antistoffen. Laboratoriumonderzoek. Laboratory Manual Laboratory manuals. Laboratory manuals. Manuels de laboratoire. Kontermann, Roland 1961-) https://id.oclc.org/worldcat/entity/E39PCjwJ3pJ8ck6XkvwPwbCwBX Dübel, Stefan Print version Antibody engineering 3540413545 (DLC) 00067931 (OCoLC)45487559 Springer lab manual